單抗144734-HRP中英文實驗說明

2019年3月，蘇州螞蟻淘生物科技有限公司正式獲得USBIo中國大陸區總代理*。USBIo全稱United States BIOLOGical，也簡稱為USBIological。位于麻省斯萬普斯科特(Swampscott, MA)。是生物化學和生物學試劑的專業生產商，產品服務于免疫學，分子生物學，分子遺傳學，體外診斷，組織培養等科研領域。產品精確度高，質量穩定，產品數量巨大，提供70,000多種抗體、抗原和生化試劑等。

主要產品種類包括：抗體、生化試劑、基因克隆相關產品、培養基、生長因子等細胞因子、分子生物學試劑。

uSBIo品牌單抗144734-HRP實驗說明

IL-17（白細胞介素-17），初由Rouvier等人鑒定為來自嚙齒動物T細胞雜交瘤的轉錄物。 1993年，是一組稱為IL-17家族的細胞因子的創始成員。在嚙齒動物中稱為CTLA8，IL-17顯示出與由T-淋巴細菌性rhADInovirus皰疹病毒saimiri的開放閱讀框編碼的病毒IL-17高度同源。小鼠IL-17與大鼠具有85％的氨基酸序列同源性。白細胞介素17是一種細胞因子，通過增加各種組織中的趨化因子產生，將單核細胞和嗜中性粒細胞募集到炎癥部位，作為延遲型反應的有效介質，類似于干擾素γ。 IL-17由T輔助細胞產生并由IL-23誘導，其導致延遲型反應中的破壞性組織損傷。白細胞介素17作為一個家族起著促炎細胞因子的作用，它可以通過細胞外病原體對免疫系統的侵襲作出反應，并誘導病原體細胞基質的破壞。白細胞介素17與腫瘤壞死因子和白細胞介素-1協同作用。為了引發其功能，IL-17結合稱為IL-17R的I型細胞表面受體，其中存在至少三種變體IL17RA，IL17RB和IL17RC。

單抗144734-HRP中英文實驗說明

IL-17(Interleukin-17), originally identified as a transcript from a rodent T-cell hybridoma by Rouvier et al. in 1993, is the founding member of a group of cytokines called the IL-17 family. Known as CTLA8 in rodents, IL-17 shows high homology to viral IL-17 encoded by an open reADIng frame of the T-lymphotropic rhADInovirus Herpesvirus saimiri. Mouse IL-17 shares 85% amino acid sequence homology with rat. Interleukin 17 is a cytokine that acts as a potent mediator in delayed-type reactions by increasing chemokine production in various tissues to recruit monocytes and neutrophils to the site of inflammation, similar to Interferon gamma. IL-17 is produced by T-helper cells and is induced by IL–23 which results in destructive tissue damage in delayed-type reactions. Interleukin 17 as a family functions as a proinflammatory cytokine that responds to the invasion of the immune system by extracellular pathogens and induces destruction of the pathogen’s cellular matrix. Interleukin 17 acts synergistically with tumor necrosis factor and interleukin-1. To elicit its functions, IL-17 binds to a type I cell surface receptor called IL-17R of which there are at least three variants IL17RA, IL17RB, and IL17RC.

UniProt Number

Q62386

Gene ID

IL17A

Applications

Suitable for use in ELISA, Western Blot.

Recommended Dilution

Optimal dilutions to be determined by the researcher.

Storage and Handling

Store at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term. Avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Immunogen

E. coli-derived mouse IL-17 recombinant protein (Position: A26-A158).

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with horserADIsh peroxidase (HRP).

Purity

Purified by immunoaffinity chromatography.

Specificity

Recognizes mouse IL-17/IL-17A. No crossreactivity with other proteins.